TEM8 (Tumor Endothelial Marker 8, Bacillus anthracis (Anthrax) Toxin Receptor, ATR1)

Tumor Endothelial Marker 8 (TEM8), also called ATR1, is a glycoprotein of the anthrax toxin receptor family that is expressed by endothelial cells. Type I transmembrane isoforms of 564 aa (80-85 kD) and 368 aa (60 kD) and potentially secreted isoforms of 330 aa and 297 aa (45 kD) are differentially expressed. All diverge at the C-terminal end but share the N-terminal vWFA domain that binds the anthrax toxin PA subunit as well as collagens I and IV. Human TEM8 shares 99% aa identity with mouse and rat and 92% identity with dog and chick TEM8 within the extracellular domain.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human TEM8 (rhTEM-8; aa 28-320: Accession # AAK52094).\n\nApplications: \nWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human TEM8. Using a colorimetric detection system, the detection limit for rhTEM8 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human TEM8. The detection limit for rhTEM8 is approximately 5 ng/well. \nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2500-05C

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Selected for its ability to detect human TEM8 in direct ELISAs and Western Blots.