Tenascin C (Tenascin-C, TNC, TN-C, TN, Cytotactin, Glioma-associated Extracellular Matrix Antigen, GMEM, GP-150-225, Hexabrachion, HXB, JI, Myotendinous Antigen, Neuronectin)

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors (Erickson and Bourdon 1989).It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).The tenascin molecule is a disulfide-linked hexamer.The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.\n\nApplications: \nSuitable for use in AF, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: In immunohistological testing of frozen tissue sections of mouse intestine, the antibody stains the core of the villi but not the epithelial cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2550-01A

Size

50ul

Applications

ELISA, IF, IHC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Ascitic fluid with 0.1% sodium azide

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

This product has been used in:Wirl G et al. Mammary epithelial cell differentiation in vitro is regulated by an interplay of EGF action and tenascin-C downregulation. J Cell Sci 108 (Pt 6):2445-56 (1995). <PUBMED:7545689>Weller A et al. Amino acid sequence of mouse tenascin and differential expression of two tenascin isoforms during embryogenesis. J Cell Biol 112:355-62 (1991). <PUBMED:1703162>Aufderheide E & Ekblom P Tenascin during gut development: appearance in the mesenchyme, shift in molecular forms, and dependence on epithelial-mesenchymal interactions. J Cell Biol 107:2341-9 (1988). <PUBMED:2461951> Maschler S et al. Enhanced tenascin-C expression and matrix deposition during Ras/TGF-beta-induced progression of mammary tumor cells. Oncogene 23:3622-33 (2004). <PUBMED:15116096>Erickson HP & Bourdon MA Tenascin: an extracellular matrix protein prominent in specialized embryonic tissues and tumors. Annu Rev Cell Biol 5:71-92 (1989). <PUBMED:2480799>Bourdon MA & Ruoslahti E Tenascin mediates cell attachment through an RGD-dependent receptor. J Cell Biol 108:1149-55 (1989). <PUBMED:2466038>

Additional Info

MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of ~210 and ~260kD. It also recognizes these tenascin chains and the 230kD endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblast lysates under reducing conditions (Aufderheide et al. 1988, Weller et al. 1991). In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I. Immunoprecipitation data suggests that molecules of the expected size for tenascin-C are detected by MTn-12, and not other tenascins. One can expect several bands in blots or immunoprecipitations but this is because the antibody can detect several splice variants of tenascin C. Nevertheless, the mapping of the epitope and the other data cannot entirely exclude the possibility that it reacts with other tenascins. This would have to be tested directly with purified tenascins. Species Crossreactivity: Crossreacts with Mouse. Not yet tested in other species.