Tenascin C (Tenascin-C, TNC, TN-C, TN, Cytotactin, Glioma-associated Extracellular Matrix Antigen, GMEM, GP-150-225, Hexabrachion, HXB, JI, Myotendinous Antigen, Neuronectin)

Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000-1:2000\nImmunohistochemistry (Paraffin/frozen): Staining of paraffin sections may be enhanced using trypsin digestion or heat mediated antigen retrieval using sodium citrate buffer pH 6.0.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman tonsil\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-14C: IgG, Fc (HRP) Pab Gt xMo\nI1904-19C: IgG, F(ab

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SPECIFICATIONS

Catalog Number

T2550-01B

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG1

References

1. Gulubova, M., Immunohistochemical localization of collagen type III and type IV, laminin, tenascin and alpha-smooth muscle actin (alphaSMA) in the human liver in peliosis. Pathol Res Pract 198: 803-812 (2002). 2. Ioachim, E. et al., Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression. Eur J Cancer 38: 2362-2370 (2002). 3. Faustino, A.M. et al., Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues. J Comp Pathol 126: 1-8 (2002). 4. Tokes, A.M. et al. Immunohistochemical localisation of tenascin in invasive ductal carcinoma of the breast. Anticancer Res 19 :175-179 (1999).

Additional Info

Recognizes human Tenascin C in both normal and hyperproliferative (neoplastic) tissues.