Testisin (Prss21, Serine Protease 21, Tryptase 4)

Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase-4. It is predicted to have substrate specificity similar to that of trypsin. Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils. Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility. Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor. Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa28-298, mouse Testisin shares 92%aa identity with rat Testisin.\n\nApplications:\nSuitable for use in Immunohistochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5mg/ml\nImmunohistochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2943-20

Size

100ug

Applications

IHC, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648. 2. Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63. 3. Inoue, M. et al. (1998) Biochem. Biophys. Res. Comm. 252:307. 4 .Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921. 5. Kawano, N. et al. (2010) Biol. Reprod. 83:359. 6. Honda, A. et al. (2002) J. Biol. Chem. 277:16976.

Additional Info

Recognizes mouse Testisin.