Testosterone, Free, Human, BioAssay(TM) ELISA Kit

The United States Biological Free Testosterone ELISA Bioassay is Competitive immunoenzymatic \ncolorimetric method for quantitative determination of Free Testosterone concentration in serum and plasma. \n \nFree Testosterone (antigen) in the sample competes with horseradish peroxidase testosterone (enzyme-labeled antigen) for binding onto the limited number of anti-testosterone (antibody) sites on the microplates (solid phase). After incubation the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB-Substrate (TMB) are added. After an appropriate time has elapsed for maximum color development, the enzyme reaction is stopped and the absorbance is determinated. Free Testosterone concentration in the sample is calculated based on a series of standards. The color intensity is inversely proportional to the Free Testosterone concentration of in the sample. Testosterone in the blood is bound to SHBG (60 %) and in lower quantities bound to other proteins. The measurement of Free Testosterone (<1% of Total Testosterone) is the only estimating of the biologically active form of this hormone. \n\nDetection Range: 0-100pg/ml \n\nSample size: 20ul \n\nPrecision: \nIntra-assay: (same/less than)10%\nInter-assay: (same/less than)10%\n\nSensitivity: 0.6pg/ml \n\nTotal Time: ~110 min\n\nKit Components: \nT2949-11A: Microtiter Plate, 1x96wells\nT2949-11B: Standard (0pg/ml), 1x1ml\nT2949-11C: Standard (0.2pg/ml), 1x1ml\nT2949-11D: Standard (1.0pg/ml), 1x1ml\nT2949-11E: Standard (4.0pg/ml), 1x1ml\nT2949-11F: Standard (20.0pg/ml), 1x1ml\nT2949-11G: Standard (100.0pg/ml), 1x1ml\nT2949-11H: Control (0.84-2.03pg/ml), 1x1ml\nT2949-11J: HRP Conjugate, 1x15ml\nT2949-11K: Wash Buffer (10X), 1x50ml\nT2949-11L: TMB Substrate, 1x15ml\nT2949-11N: Stop Solution (0.15M H2SO4), 1x15ml \n\nStorage and Stability:\nStore all components at 4 degrees C in the dark. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Diluted Wash Buffer is stable for 30 days at 4 degrees C.

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SPECIFICATIONS

Catalog Number

T2949-11

Size

96Tests

Applications

ELISA

References

1. McCann, D. et. al., Clin. Immunoassay 8:234-6 (1985). 2. Ekins, R.P., J. Clin. Immunoassay (1984) 7: 163-180. 3. Paulson, J.D., et. al., Am. J Obst. Gynecol (1977) 128: 851-857. 4. Odlind, V. et. al., Clin. Endocrinology (1982) 16: 243-249. 5. Green, P.J., Clin Chem (1982) 28: 1237. 6. Wu, C.H., Obstet Gynecol. (1982) 60: 188-194.