Testosterone, Human, BioAssay(TM) ELISA Kit

The United States Biological Testosterone, Human, BioAssay(TM) ELISA Kit is for the Quantitative Determination of Testosterone Concentration in Human Serum. Testosterone (17B-hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the B position at C-17. This steroid hormone has a molecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in samples with the following disease; Hypopituitarism, Klinefelter's syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases.The United States Biological Testosterone, Human, BioAssay(TM) ELISA Kit are designed for the measurement of total Testosterone in sample serum. \n\nKit Components:\n1. Microtiter Plate: 1x96 wells\n2. Testosterone Reference Standards: 6X500ul (0, 0.1, 0.5, 2, 6, 18ng/ml) \n3. Rabbit Anti-Testosterone Reagent: 1X7ml \n4. Testosterone (HRP) Reagent: 1X12ml \n5. Control 1, liquid: 1X500ul\n6. Control 2, liquid: 1X500ul \n7. Tetramethylbenzidine (TMB): 1x11ml\n8. Stop Solution (1N HCl), 11ml.\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T2949-H

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Chen, A., et al., Fertil. Steril., 1991; 55: 1202-1203. 2. Granoff, A.B. and Abraham, G.E., Peripheral and adrenal venous levels of steroids in a sample with virilizing adrenal adenoma. Obstet. Gynecol., 1979; 53:111-115. 3. Bricaire, C., Raynaud, A., Benotmane, A., et al., Selective venous catheterization in the evaluation of hyperandrogenism. J. Endocrinol Invest., 1991; 14: 949-956. 4. Heinonen, P.K., Androgen production by epithelial ovarian tumours in post-menopausal women. Maturitas, 1991; 13: 117-117-122. 5. Tietz, N.W. ed., Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Co., Philadelphia, 1995: 578-580. 6. USA Center for Disease Control/National Institute of Health Manual,