Tetracycline, amide (HRP)

Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability. \n\nTetracyclines are variably absorbed after oral administration and penetrate into most tissues and body fluids, with the highest levels in kidney and liver. All tetracyclines are excreted in urine and faeces, either as the parent molecule or as microbiologically inactive forms. The inactive 4-epimers (33) are thought to be artefacts produced by chemical rather than biological processes and can form spontaneously during sample preparation (32). Tetracyclines are used extensively in veterinary medicine and their use in food producing animals could result in potentially harmful concentrations in tissue, organs and milk. The potential risk is reduced by withdrawal of the drug for a fixed period before slaughter, although residual levels may remain.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:20 when used in a competitive ELISA with antibody T2965-02.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2965-01A

Size

500ul

Applications

ELISA

Form

Supplied as a liquid. Labeled with horseradish peroxidase (HRP). Only dilute the concentrate as required and use the working strength conjugate immediately after dilution.

Purity

Purified