Tetracycline Hydrochloride

Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability. Applications: Recommended for use in cell culture applications. Recommended Dilutions: Cell Culture: 10mg/L. Stable at 37 degrees C for 4 days. Solubility: Water soluble at 5-10g/100ml Conforms to the following USP28 References: Appearance: Yellow crystalline powder Purity: 95-105% Potency (Assay): (same/more than) 900ug/mg Identification A (IR): Conforms Identification B (UV): Conforms Identification C (HPLC): Conforms Crystallinity: Conforms Loss on Drying: (same/less than) 2.0% Heavy Metals: (same/less than) 0.005% 4-Epianhydrotetracycline: (same/less than) 2% Anhydrotetracycline: (same/less than) 0.5% pH (10mg/ml): 1.8-2.8 Specific Optical Rotation: -240-255 degrees Individual Related Substance: (same/less than) 3% Total Unknown Related Substance: (same/less than) 5% Bulk Density: 0.42-0.55g/ml Residual Solvent: acetone: (same/less than) 0.02% n-butanol: (same/less than) 0.2% Tallow derivatives: Produced on the basis of chemical synthetic process; no any animal material contained during the whole production process and also no raw materials are used which are generated from animals during production. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O. Filter sterilize, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. Further dilutions can be made in assay buffer.