Tetracycline Repressor (TetR)

Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes. It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic). Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit. Mode of resistance is the loss of cell wall permeability.\n\nThe 23kD Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2965-35

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Yeast/Fun

Form

Supplied as a liquid in PBS, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Pook, E., et al., Eur. J. Biochem. 258:915-922 (1998)2. Hillen, W. and Berens, C., Annu. Rev. Microbiol. 48:345-369 (1994)3. Gatz, C., et al., Biospektrum 1:23-29 (1995)4. Mayford, M., et al., Science 274:1678 (19965. Angew Chem Int Ed Engl. 2000 Jun 16;39(12):2042-2052

Additional Info

Recognizes the TetR portion of bait fusion protein in the yeast two-hybrid system. Detects a band of ~37kD by Western blot.