Tetrahydrocannabinol, D9 (THC)

Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis. Cannabis affect cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. D9-THC is also immunosuppressive and impairs cell-mediated immunity, humoral immunity and cellular defenses against a variety of infectious agents both in vivo and in vitro. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoids, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10K-100K; using 50-100 ng of THC-BSA or other protein conjugates/well\nWestern Blot: 1-10ug/ml (ECL)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2970-05

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a lyophilized powder from 10mM Tris-saline buffer, pH 7.5, 0.2% BSA, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography

Isotype

IgG1

References

1. Hunt CA et al (1980) J. Pharmacol. Exp. Ther. 215: 35. 2. Wall ME (1976) in \"metablosim of Cannabinoid in Man\" Braude MC and Szara S, Eds, Raven Press, 1:93-116. 3. Kanter Sl et al (1974) Clin. Chem. 20: 860.

Additional Info

Recognizes THC. Species Crossreactivity: all species