Tetratricopeptide Repeat Domain 5 (TTC5)

Description:\nTetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats (1). Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a \nright-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those \nseen with protein chaperones HSP70 and HSP90 (2) and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5 (3), suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 05-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRAW264.7 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T3503-15

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.\n

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

References

1. Blatch GL and Lassle M. The tetratricopeptide repeat: a structural motif mediating protein-protein interactions. BioEssays 1999; 21:932-9. 2. Song Y and Masison DC. Independent regulation of Hsp70 and Hsp90 chaperones by Hsp70/Hsp90-organizing protein Sti1 (Hop1). J. Biol. Chem. 2005; 280:34178-85. 3. Yang J, Roe SM, Cliff MJ, et al. Molecular basis for TPR domain-mediated regulation of protein phosphatase 5. EMBO J. 2005; 24:1-10. (WD1105)

Additional Info

Recognizes human TTC5. Species Crossreactivity: mouse.