The Tetro One-Step RT-PCR Kit has been designed for highly sensitive one-step RT-PCR reactions using any RNA template. The kit employs an enzyme formulation, which includes IMMOLASE(TM) (our widely used hot-start DNA polymerase) and MMLV reverse transcriptase. The Tetro One-Step RT-PCR Kit provides highly specific reverse transcription and PCR in a single tube, using gene-specific primers on either total RNA or mRNA. The kit is provided with RNase inhibitor to protect template RNA from degradation. The proprietary buffer is highly optimized and balanced, leading to outstanding results. The kit is ideal for the synthesis of double-stranded cDNA products for subsequent cloning, sequencing, expression, or transcription analysis. The kit can be used with starting amounts of RNA template from 100pg to 2_g.
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