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TFB2M, Recombinant, Human, aa20-396, His-Tag (Dimethyladenosine Transferase 2 Mitochondrial, Hkp1, mtTFB2)

Cat no: 138757

TFB2M, Recombinant, Human, aa20-396, His-Tag (Dimethyladenosine Transferase 2 Mitochondrial, Hkp1, mtTFB2)

TFB2M is S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. This protein is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. TFB2M stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. \n\nSource:\nRecombinant protein corresponding to aa20-396 from human TFB2M at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~45.8kD (401aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSM QIYLGKPSRP PHLLLECNPG PGILTQALLE AGAKVVALES DKTFIPHLES LGKNLDGKLR VIHCDFFKLD PRSGGVIKPP AMSSRGLFKN AGRFCI LGSEAATRKH LPARNHCGLS DSSPQLWPEP DFRNPPRKAS KASLDFKRYV TDRRLAETLA\nLGIEAVPWTA DIPLKVVGMF PSRGEKRALW KLAYDLYSCT SIYKFGRIEV NMFIGEKEFQ KLMADPGNPD LYHVLSVIWQ LACEIKVLHM EPWSSFDIYT RKGPLENPKR RELLDQLQQK LYLIQMIPRQ NLFTKNLTPM NYNIFFHLLK HCFGRRSATV IDHLRSLTPL DARDILMQIG KQEDEKVVNM HPQDFKTLFE TIERSKDCAY KWLYDETLED R\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138757

Size

50ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Litonin,D.,et al. (2010) J. Biol. Chem. 285(24), 18129-18133 2. Norrbom,J.,et al. (2010) Acta Physiol (Oxf) 198(1), 71-79.

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