TFF1/pS2 (Trefoil Factor 1)

The trefoil factor family of proteins (trefoil factor 1 [TFF1]/pS2, TFF2, and TFF3) are a group of highly conserved, thermostable, and protease resistant polypeptides. The family was named for the disulfide bond configuration of the trefoil, or P domain, which forms a three-leaved structure analogous to a trefoil or clover leaf. Each trefoil domain comprises 42-43 amino acids containing six cysteine residues that form disulfide bonds, resulting in the characteristic trefoil structure (1,2). The trefoil peptides are localized within mucous granules in mucous-secreting cells and are expressed and secreted by epithelial cells that line mucous membranes (2).\nTFF1 is expressed predominantly by the gastric epithelia, in the upper portion of the glandular pits, and is highly expressed in some adenocarcinomas such as breast cancer (1,3,4). In the context of breast cancer, TFF1 is highly expressed in estrogen receptor-positive tumors. Indeed, TFF1 expression is directly regulated by estrogen receptor alpha (4). In the stomach, secreted TFF1 is a component of the protective mucous layer. TFF1 expression is strongly induced after mucosal injury (5) and is involved in stomach ontogenesis and maintenance of the integrity of the mucosa (1,3). Research studies have shown frequent loss of TFF1 expression in more than two-thirds of gastric carcinomas resulting from mutation-independent mechanisms (6-8).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137500

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Thim, L. and May, F.E. (2005) Cell Mol Life Sci 62, 2956-73. 2. Taupin, D. and Podolsky, D.K. (2003) Nat Rev Mol Cell Biol 4, 721-32. 3. Ribieras, S. et al. (1998) Biochim Biophys Acta 1378, F61-77. 4. Corte, M.D. et al. (2006) Breast Cancer Res Treat 96, 63-72. 5. Taupin, D. et al. (2001) Lab Invest 81, 397-408. 6. Carvalho, R. et al. (2002) Lab Invest 82, 1319-26. 7. Katoh, M. (2003) Int J Mol Med 12, 3-9. 8. McChesney, P.A. et al. (2006) Cancer Res 66, 1346-53.

Additional Info

Recognizes endogenous levels of total human TFF1/pS2. Reacts with precursor and mature forms of TFF1/pS2. Not predicted to React with TFF2 or TFF3. Species Crossreactivity: mouse.