TGase 2 (Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), G-ALPHA-h, GNAH, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGM2, TGase C, TGase H, TGC, Tissue transglutaminase, Transglutaminase-2, Transglutaminas

TGase II is implicated in programmed cell death, signal transduction, drugresistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing. \nExcellent for staining of endothelial cells in mouse tissues.\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nImmunoprecipitation (Native verified): 10ug/mg protein lysate. Use Protein A. \nWestern Blot: 5-10ug/ml for 2hrs at RT \nImmunohistochemistry (Formalin/paraffin): 5-10ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. \nOptimal dilutions to be determined by researcher.\n\nPositive Control: \nHUVEC cells. Breast carcinoma. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8260-01X

Size

500ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See T8260-01.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Greenberg, C.S., et. al., Faseb Journal, 1991 Dec, 5(15): 3071-7. 2. Lee, K.N.., et. al. Biochemical and Biophysical Research Communications, 1989, 162(3): 1370-5. 3. Birckbichler, P.J., Patterson, M.K. Jr. Cellular transglutaminase, growth and transformation. Annals of the New York Acad of Sciences, 1978, 312: 35465. 4. Folk, J.E., Finlayson, J.S. The epsilon-(gamma-glutamyl)lysine crosslink and the catalytic role of transglutaminases. Advances in Protein Chemistry, 1977, 31:1-133. 5. Aeshlimann, D., Paulson, M., Thromb Haemost, 1994, 71: 402-415. 6. Gentile, V., Davies, P.J., Baldini, A. The human tissue transglutaminase gene maps on chromosome 20q12 by in situ fluorescence hybridization. Genomics, 1994, Mar 15, 20(2): 295-297. 7 Mehta, K., Lopez-Berestein, G. Expression of tissue transglutaminase in cultured monocytic leukemia (THP-1) cells during differentiation. Cancer Research, 1986, Mar, 46(3: 1388-1394. 8. Chiocca, E.A., Davies, P.J., Stein, J.P. The molecular basis of retinoic acid action. Transcriptional regulation of tissue transglutaminase gene expression in macrophages. Journal of Biological Chemistry, 1988, Aug 15, 263(23) :11584-11589. 9. Suto, N., Ikura, K., Sasaki, R. Expression induced by interleukin-6 of tissue-type transglutaminase in human hepatoblastoma HepG2 cells. Journal of Biological Chemistry, 1993, Apr 5, 268(10): 7469-7473. 10. George, M.D., Vollberg, T.M., Floyd, E.E., Stein, J.P., Jetten, A.M. Regulation of transglutaminase type II by transforming growth factor-beta 1 in normal and transformed human epidermal keratinocytes. Journal of Biological Chemistry, 1990, 265(19): 11098-11104. 11. Piacentini, M., Autori, F., Dini, L., et. al. Cell Tissue Research, 1991, 263: 227

Additional Info

Recognizes human Transglutaminase II. Species Crossreactivity: mouse and rat.