TGF beta 1 BioAssay(TM) ELISA Kit, Rat

Sandwich ELISA quantitative detection kit for Rat TGF beta 1 in cell culture supernates, serum, plasma (EDTA) and urine.\n\nTransforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.1 TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.2 TGFbeta1 is also an important mediator of bone remodeling.3 TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.4 TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.5 TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.6 The standard product used in this kit is recombinant TGFb1 with the molecular mass of 25kD.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is cross-reactivates with TGFb2, TGFb3, TGFb5 <1%.\n\nUniprot ID:\nP17246\n\nEntrez Gene ID:\n59086\n\nKit Components:\n96-well plate pre-coated with anti-TGF beta 1, Rat\nLyophilized recombinant TGF beta 1, Rat standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144052

Size

96Tests

References

1. Sporn, M. B.; Roberts, A. B.; Wakefield, L. M.; Assoian, R. K. Transforming growth factor-beta: biological function and chemical structure. Science 233: 532-534, 1986. \n2. Blanchette, F.; Day, R.; Dong, W.; Laprise, M.-H.; Dubois, C. M. TGF-beta-1 regulates gene expression of its own converting enzyme furin. J. Clin. Invest. 99: 1974-1983, 1997. \n3. Janssens, K.; Gershoni-Baruch, R.; Guanabens, N.; Migone, N.; Ralston, S.; Bonduelle, M.; Lissens, W.; Van Maldergem, L.; Vanhoenacker, F.; Verbruggen, L.; Van Hul, W. Mutations in the gene encoding the latency-associated peptide of TGF-beta-1 cause Camurati-Engelmann disease. Nature Genet. 26: 273-275, 2000. \n4. Li, A. G.; Wang, D.; Feng, X.-H.; Wang, X.-J. Latent TGF-beta-1 overexpression in keratinocytes results in a severe psoriasis-like skin disorder. EMBO J. 23: 1770-1781, 2004. \n5. Li, B.; Khanna, A.; Sharma, V.; Singh, T.; Suthanthiran, M.; August, P. TGF-beta-1 DNA polymorphisms, protein levels, and blood pressure. Hypertension 33: 271-275, 1999. \n6. Saito, T.; Kinoshita, A.; Yoshiura, K.; Makita, Y.; Wakui, K.; Honke, K.; Niikawa, N.; Taniguchi, N. Domain-specific mutations of a transforming growth factor (TGF)-beta 1 latency-associated peptide cause Camurati-Engelmann disease because of the formation of a constitutively active form of TGF-beta 1. J. Biol. Chem. 276: 11469-11472, 2001. \n