Transforming growth factor beta (TGF-b) is a member of the TGF family of growth factors involved in the regulation of a variety of cellular activities. In mammals, TGF-b1 regulates reproduction, and ovarian function in particular. Homologs of human TGF-b3 have been identified in other vertebrate species, but have not been well characterized in zebrafish. Zebrafish TGF-b3 transcripts were first detected in the notochord, followed by expression in the developing pharyngeal arch and neurocranial cartilage. Expression in the pectoral fins of zebrafish, but not reported in the mammalian forelimb ortholog, suggests a novel function of TGF-b3 in early fish development.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.
Control Peptide: T8250-27Q
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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