Transglutaminase (TGase) catalyses the crosslink of proteins by forming epsilon-(Upsilon-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. In mammals, eight distinct TGase isoenzymes have been identified.Tissue transglutaminase (tau TGase), also known as TGase 2, has four distinct domains: N-terminal beta-sandwich, catalytic core and two C-terminal beta-barrel domains. tTGase may have a role in cell death, cell proliferation, cell differentiation, and receptor-mediated endocytosis. In the Alzheimer's disease brain, the elevated tau TGase activity is manifested by polymerization of a number of proteins,including A-beta peptide, beta-amyloid precursor protein and the tau protein, with formationof neurofibrillary tangles.
Applications:
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500
Immunoprecipitation: 1-2ul/400ul cell lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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