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Thiamine, Conjugated

Cat no: T5004

Thiamine, Conjugated

Thiamin is one of the B vitamins, a group of water-soluble vitamins that are part of many of the chemical reactions in the body. Thiamin (vitamin B1) helps the body's cells convert carbohydrates into energy. It is also essential for the functioning of the heart, muscles, and nervous system.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:500-1:1000. Immunoreactive fibers located in the pulvinar nucleus of monkey brain. Also, immunoreactive fibers located between the pulvinar and caudate nucleus of monkey brain.\nOptimal dilutions to be determined by the researcher.\n\nSpecificity:\nUsing a conjugate Thiamine-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :\n\nCompound Cross-reactivity ratio (a)\nThiamine-BSA 1\nFolic acid-BSA 1/>80\nThioctic acid-BSA 1/>50,000\n\n(a) : Thiamine-BSA concentration/unconjugated or conjugated compound concentration at half displacement.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5004

Size

100ug

Applications

ELISA, IHC

Hosts

Rat

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Mangas A., et al., Life Sci. 79(12):1121-8 (2006)

Additional Info

Recognizes protein-linked Thiamine. See additional crossreactivity data.

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