Free thiols in biological systems have important roles. Oxidatively modified thiol groups of cysteine residues are known to modulate the activity of a growing number of proteins. One of the most pressing problems with this approach is to accurately determine the extent of modification of specific amino acids, such as cysteine residues, in a complex protein sample.
This Kit allows users to accurately determine the extent of free thiol content in samples using a proprietary nonfluorescent substrate that is converted to a brightly fluorescent product upon reaction with the thiol in the sample. The thiol in the sample can either be one that is generated by a reaction, such as the end product of an enzymatic reaction such as glutathione, or can be the cysteine content of the protein to be measured.
Almost any sample containing free SH goups can be measured in the kit by diluting into the supplied Diluent. The kit has been validated using both glutathione and cysteine.
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