Thioredoxin, Recombinant, Human

Thioredoxins are small disulphide -containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. This could be especially useful in refolding proteins expressed in E. coli. To this end, thioredoxin has been shown to act as a protein disulfide isomerase.\n\nRecombinant human Thioredoxin is a single, non-glycosylated, polypeptide chain of 96aa having a molecular mass of 11.9kD. The pI is 4.67.\n\nBiological Activity:\nThe specific activity was found to be ~3IU/mg. Activity was assayed by measuring the change in absorbance at 650nm at 25 degrees C using 0.13uM bovine insulin containing 0.33mM DTT (pH 6.5).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5010-11

Size

20ug

Form

Supplied as a lyophilized powder in 20mM PBS, pH 7.4. Reconstitute in sterile ddH2O. Can be further diluted to other aqueous solutions.

Purity

~ 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (1EU/ug)

References

1. Kondo, N., et al., J. Immunol. 172(1): 442-448 (2004). 2. Liu, F., et al., Gene 315: 71-78 (2003). 3. Watson, W.H., et al., J. Biol. Chem. 278(35): 33,408-33,415 (2003). 4. Jimenez, A. & Miranda-Vizuete, A., Protein Expr. Purif. 27(2): 319-324 (2003). 5. Sadek, C.M., et al., J. Biol. Chem. 278(15): 13,133-13,142 (2003). 6. Mitsui, A., et al., Antioxid. Redox. Signal 4(4): 693-696 (2002).