Thioredoxin, Recombinant, Human (TRDX, TRX 1, TRX1, TRX-1, TRX, TXN, TXN Protein, ATL-derived Factor, ADF, DKFZp686B1993, MGC61975, Surface-associated Sulphydryl Protein, SASP)

Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant TXN protein, fused to His-tag at N-terminus, was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-105 of human His tag at the N-terminus expressed in E. coli.\n\nAmino Acid Sequence:\nMGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nActivity Assay:\n1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% insulin, 2mM EDTA.\n2. Add 4ug of T5010-02H into reaction cocktail and mix immediately. \n3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.\n\nBiological Activity:\nSpecific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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