Thioredoxin, Recombinant, Yeast

Thioredoxins are a group of ~12kD redox proteins that mediate numerous cytosolic processes in all cells. Human thioredoxin can be exported out of the cell where it has additional functions including the ability to stimulate cell growth.\n\nRecombinant yeast Thioredoxin is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity:\nThe specific activity was found to be ~3IU/mg.\n\nUnit Definition:\nOne unit equals the change of A=1 unit in absorbance at 650 nm at 25 degrees C using 0.13uM bovine insulin containing 0.33mM DTT (pH 6.5).\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Thioredoxin Reductase\n\nReconstitution:\nReconstitute the lyophilized Thioredoxin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5010-11A

Size

5ug

Form

Supplied as a lyophilized powder in 20mM phosphate buffer, pH 7.4.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Yeast thioredoxin peroxidase expression enhances the resistance of Escherichia coli to oxidative stress induced by singlet oxygen. Kim SY, Kim EJ, Redox Rep 2002;7(2):79-84 2. Localization of TDPX1, a human homologue of the yeast thioredoxin-dependent peroxide reductase gene (TPX), to chromosome 13q12. Pahl P, Berger R, Chae HZ, Genomics 1995 Apr 10;26(3):602-6 3. Deoxyribonucleotides are maintained at normal levels in a yeast thioredoxin mutant defective in DNA synthesis. Muller EG, J Biol Chem 1994 Sep 30;269(39):24466-71 4. Yeast thioredoxin genes. Gan ZR, J Biol Chem 1991 Jan 25;266(3):1692-6 5. Purification and characterization of yeast thioredoxin reductase. Speranza ML, Ronchi S, Biochim Biophys Acta 1973 Dec 19;327(2):274-81 6. Yeast thioredoxin. Amino-acid sequence around the active-center disulfide of thioredoxin I and II. Hall DE, Baldesten A, Reichard P, Eur J Biochem 1971 Nov 11;23(2):328-35.