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Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB)

Cat no: T5010-14E

Thioredoxin Reductase, Recombinant, E. coli (TRXR, trxB)

TRXB(Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E.coli TRXB protein was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-321 of human TRXB 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI KELPELWLGQ NEFDFMTDFV CKQQPSRVSC LEHHHHHH\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5010-14E

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.

Purity

~ 90% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Faulkner MJ., et al. (2008) Proc Natl Acad Sci U S A. 105(18):6735-40. 2. Takahata M., et al. (2008) J Biochem. 143(4):467-73.

Alternative Names

Thioredoxin Reductase, FAD/NAD(P)-binding, EC=1.8.1.9

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