THOC4 (ALY, Transcriptional Coactivator Aly/REF)

mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing of mRNAs and the packaging of mRNAs in the form of mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY via its interaction with the splicing factor UAP56, forming a large complex termed transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus to the cytoplasm (1-3,6).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence(Immunocytochemistry): 1:400\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137502

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Katahira, J. (2012) Biochim Biophys Acta 1819, 507-13. 2. Siddiqui, N. and Borden, K.L. (2012) Wiley Interdiscip Rev RNA 3, 13-25. 3. Reed, R. and Magni, K. (2001) Nat Cell Biol 3, E201-4. 4. Masuda, S. et al. (2005) Genes Dev 19, 1512-7. 5. Luo, M.L. et al. (2001) Nature 413, 644-7. 6. Kang, Y. and Cullen, B.R. (1999) Genes Dev 13, 1126-39.

Additional Info

Recognizes endogenous levels of total human THOC4/ALY. Species Crossreactivity: mouse, rat, monkey.