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THP-1 Nuclear Extract, PMA stimulated (Human Monocyte)

Cat no: T5019-01A

THP-1 Nuclear Extract, PMA stimulated (Human Monocyte)

Permanent murine cell lines of fast and slow skeletal myocyte derivation.\n\nNuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with PMA (10ng/ml).\n\nStorage and Stability\nFor long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

T5019-01A

Size

200ug

Applications

WB

Reactivities

Hum

Form

Supplied as a liquid in 20mM Hepes pH 7.5, 400mM NaCl, 20% glycerol, 0.1 mM EDTA, 10mM NaF, 10uM Na2MoO4, 1mM NaVO3, 10mM PNPP, 10mM beta-glycerophosphate, 1mM DTT and protease inhibitors.

Purity

Extracts have been quality control tested by Western blot.

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