Threonine, Phosphorylated (Biotin)

Supplier:

United States Biological

Cat no: T5020-05

Threonine, Phosphorylated (Biotin)

Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000-1:5000\nWestern Blot: 1:500-1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5020-05

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. Reconstitute with 100ul sterile PBS.

P Type

Mab

Purity

Purified

Isotype

IgG

References

1. Hunter, T. (1985) Annu. Rev. Biochem., 54, 897. 2. Heffetz, D. (1991) Enzymol., 201, 44. 3. Alexander, D.et al (1989) Immunol. Today, 10, 200. 4. Levine, L., et al., (1989) J. Immunol. Methods, 124, 239.

Additional Info

Recognizes phosphorylated threonine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP, or ATP.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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