Thrombin

Supplier:

United States Biological

Cat no: T5045-01C

Thrombin

Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfide bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin (1). The molecular mass of thrombin is 37kD.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:25, using thrombin directly coated onto plate\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n

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SPECIFICATIONS

Catalog Number

T5045-01C

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG1,k

References

1. Fenton JW 2nd, Bing DH (1986) Thrombin active-site regions. Sem Thromb Hemost 12:200-208.\n2. Skrzypczak-Jankun E, Carperos V E, Ravichandran K G, Tulinsky A, Westbrook M, Maraganore J M (1991) Structure of the Hirugen and Hirolog 1 complexes of alfa-Thrombin. J.Mol.Biol. 221, 1378-1393\n3. Rydel T J, Tulinsky A, Bode W, Huber R (1991) Refined structure of the Hirudin-Thrombin Complex (1991) J. Mol. Biol. 221, 283-601

Additional Info

Recognizes human thrombin. Also recognizes complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with pro-thrombin. A strong reaction is seen in ELISA with thrombin directly coated onto the microtiterplate. Biotinylation of thrombin blocks antibody binding.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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