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Thrombopoietin, Recombinant, Mouse (THPO, TPO, C-mpl Ligand, ML, Megakaryocyte Colony-stimulating Factor, MKCSF, Megakaryocyte Growth and Development Factor, MGDF, MGC163194, MPLLG, Myeloproliferative Leukemia Virus Oncogene Ligand)

Cat no: T5050-12A

Thrombopoietin, Recombinant, Mouse (THPO, TPO, C-mpl Ligand, ML, Megakaryocyte Colony-stimulating Factor, MKCSF, Megakaryocyte Growth and Development Factor, MGDF, MGC163194, MPLLG, Myeloproliferative Leukemia Virus Oncogene Ligand)

TPO (Thrombopoietin, also known as Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulation levels of platelets in vivo. The murine TPO is a soluble 18.7kD protein containing 174 amino acid residues, which comprises the receptor binding domain of the Mpl-ligand protein.\n\nSource:\nRecombinant mouse Thrombopoietin expressed in E. coli.\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells is < 1.0ng/ml, corresponding to a specific activity of > 1x10e6 units/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5050-12A

Size

50ug

Form

Supplied as a lyophilized powder from 10mM Tris, pH 8.0. Reconstitute with sterile ddH20 to a concentration of 0.5-1mg/ml.

Purity

~98% (SDS-PAGE and HPLC). Endotoxin: (same/less than) 0.1ng/ug.

Alternative Names

m-TPO, rm-TPO

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