Thrombospondin, Human

Thrombospondin is a high-molecular weight, heparin-binding glycoprotein constituent of human platelets. Ranging from 30-50ug per 109 platelets, thrombospondin is one of the most abundant proteins in the platelet a-granule. Thrombospondin was initially termed "thrombin-sensitive protein" based upon its release by thrombin-activated platelets. Structurally, thrombospondin is a 450,000 MW glycoprotein consisting of three, identical, disulfide-linked polypeptide chains. The binding of thrombospondin to the surface of both resting and thrombin-activated platelets has been reported. A thrombospondin-specific membrane receptor has also been partially characterized. Functionally, platelet-derived thrombospondin may play a role in platelet adherence and aggregation. Thrombospondin is not an exclusive product of platelets and megakaryoctes. The synthesis of thrombospondin by endothelial cells, fibroblasts, monocytes and macrophages, and osteoblasts has been reported. Thrombospondin is also an integral component of the basement membrane in a number of different tissues. Thrombospondin interacts with a variety of extracellular macromolecules including heparin, collagen, fibrinogen and fibronectin, plasminogen, plasminogen activator, and osteonectin.Through the collective efforts of a number of different investigators employing both peptide chemistry and cDNA analytical techniques, distinct heparin, Ca2+-ion, platelet, and protein binding domains within thrombospondin have been identified. Based upon its specific interactions with both cells and extracellular matrix components, thrombospondin has been hypothesized to be a member of a class of extracellular proteins which may modulate cell-matrix interaction.\n\nSource:\nHuman thrombospondin, from Human Platelet\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5110-01

Size

25ug

Form

Supplied as a lyophilized powder from 20mM Tris, pH 8, 600mM sodium chloride, 1mM calcium chloride, 20% sucrose. Reconstitute with 102.1ul sterile ddH2O.

Purity

(same/more than)95% pure (SDS-PAGE)