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Thrombospondin Type-1 Domain-containing Protein 1, Recombinant, Mouse (THSD1, Transmembrane Molecule with Thrombospondin Module, Tmtsp)

Cat no: T5101-10

Thrombospondin Type-1 Domain-containing Protein 1, Recombinant, Mouse (THSD1, Transmembrane Molecule with Thrombospondin Module, Tmtsp)

Mouse THSD1 (Thrombospondin type-1 domain-containing protein 1), also known as Transmembrane molecule with thrombospondin module (Tmtsp), is a 95kD, type I transmembrane protein (1). It is synthesized as a precursor that is 851 amino acids (aa) in length, and has a 24 aa signal sequence, a 388 aa extracellular region, a 21 aa transmembrane region, and a 418 aa cytoplasmic region. The extracellular region contains six potential N-linked glycosylation sites and the thrombospondin type-1 (TSP1) domain, which is implicated in cell adhesion and migration (1). Also present in the molecule are 9 PKC-phosphorylation sites, 3 Ig-like domains, and a tyrosine-phosphorylation site by its C-terminus (1). Mouse THSD1 shares 78% aa sequence identity with human THSD1. THSD1 is highly expressed in hematopoietic stem cells (HSCs) and progenitor cells, including CD34-/lowKit+Sca-1+Lin- HSCs, CD34+Kit+Sca-1+Lin- and Lin-progenitor cells, and to a lesser degree in thymic CD4-CD8- cells (1). Expression gradually decreases during T cell differentiation (1). In addition, THSD1 is widely expressed on endothelial cells, with highest expression in the lung (1). THSD1

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SPECIFICATIONS

Catalog Number

T5101-10

Size

50ug

Applications

WB

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile PBS.

Purity

(same/more than)90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.\nEndotoxin: (same/less than)1EU/ug protein (LAL)

References

1. Takayanagi, S. et al., 2006, Blood 107:4317.

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