Thymic Stromal Lymphopoietin Protein Receptor (Cytokine Receptor-Like 2, TSLP-R)

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells; cotransfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex. This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1. Despite its predicted molecular weight, TSLP-R often migrates at a higher molecular weight in SDS-PAGE.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman liver tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5150-07

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ziegler, S.F. and Liu, Y.-J. (2006) Nature Immunol. 7:709-714. 2. Sims, J.E., et al. (2000) J. Exp. Med. 192:671-680. 3. Levin, S.D., et al. (1999) J. Immunol. 162:677-683. 4. Park, L.S., et al. (2000) J. Exp. Med. 192:659-670. 5. Isaksen, D.E., et al. J. Immunol. 163:5971-5977.

Additional Info

Recognizes human Thymic Stromal Lymphopoietin Protein Receptor.