Thymidine Phosphorylase (TYMP, ECGF1, Gliostatin, hPD-ECGF, MNGIE, PDECGF, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase, TP)

Thymidine phosphorylase (TP) is a platelet-derived endothelial cell growth factor (PD-ECGF) that \ncatalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate (1). This intracellular enzyme is capable of both promoting angio-genesis and inhibiting apoptosis. Thymidine phosphorylase catalytic activity is required for its angiogenic function (2,3). Increased expression of TP/PD-ECGF is seen in a wide vari-ety of different solid tumors and inflammatory diseases and is often associated with poor prognosis (4,5). Alternatively, TP can activate fluorouracil derivative (DFUR) prodrugs and increase the antitumor activity of the related treatment (1,5). The use of thymidine phosphorylase as a cancer therapeutic target has been studied extensively, with emphasis on either inhibiting TP enzymatic activity or increasing enzyme induc-tion with concomitant DFUR treatment (1,5). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5152-10D

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 0.02% sodium azide and 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

1. Rooseboom, M. et al. (2004) Pharmacol Rev 56, 53-102, 2. Moghaddam, A. and Bicknell, R. (1992) Biochemistry 31, 12141-6, 3. Furukawa, T. et al. (1992) Nature 356, 668, 4. Toi, M. et al. (2005) Lancet Oncol 6, 158-66, 5. Liekens, S. et al. (2007) Biochem Pharmacol 74,1555-67.

Additional Info

Recongnizes endogenous levels of human total TP/ECGF1 protein.