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Thymidylate synthase, aa1-313, Recombinant Human, His-Tag (TS)

Cat no: T5156

Thymidylate synthase, aa1-313, Recombinant Human, His-Tag (TS)

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP(thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nRecombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli\n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ \nLQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5156

Size

100ug

Form

Supplied as a liquid in 20 mM Tris-HCl, pH8.0, 1mM DTT 10% glycerol

Purity

~95% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Chiu TH., et al. (2009) Anticancer Res. 29(11):4503-11. 2.Pe

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