Thymosin B10

Thymosin beta 10 (TB10) is a 6.5kD member of the Bthymosin family of molecules. Members of this family range from 41-44aa in length, and possess an isoelectric point that lies between pH 5.07.0. Multiple cell types produce TB10, either constitutively, or after stimulation. They include monocytes/macrophages, endothelial cells, thyroid epithelium, spermatids, fetal cerebellar Purkinje cells plus renal tubule epithelium, and multiple tumor types. TB10 is\nprincipally an intracellular molecule. It forms a 1:1 complex with Gactin and blocks Factin\npolymerization. This regulates the availablility of actin monomers for filament formation and subsequent cell migration. It is also known to be noncanonically secreted, acting on endothelium and blocking angiogenesis. Mature human TB10 is 43aa in length (aa244) It contains an actinbinding site (aa1823), an Nterminal acetylated alanine, four acetylated lysines (#4?15?\n26? 39), and one phosphorylation site at Thr23. Mature TB10 is identical to mouse TB10 in aa sequence, and it shares 74% aa sequence identity with its human family member TB4. Human Thymosin B10.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1mg/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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