Thyroglobulin (Tg)

Supplier:

United States Biological

Cat no: T5300-11

Thyroglobulin (Tg)

Human Thyroglobulin (Tg) is a 660kD, dimeric protein produced by and used entirely within the thyroid gland. Human Thyroglobulin Tg was referred to as 'colloid'. Human Thyroglobulin (Tg) is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine. Patients with Hashimoto's thyroiditis or Graves' disease, frequently develop antibodies against human Thyroglobulin (Tg). Human Thyroglobulin Tg-specific antibodies help in the diagnosis of these diseases, but they also may be present in apparently healthy euthyroid individuals. Human Thyroglobulin (Tg) levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. Thyroglobulin (Tg) levels in the blood can also be elevated in cases of Graves disease.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistology: (Formalin/paraffin): 1:125-1:250 for 30 min at RT. No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nThyroid\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5300-11

Size

100ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified.

References

1. Burt AD et al. J Clin Pathol. 1985; 38:893-6.\n2. Kastrup J et al. Scand J Clin Lab Invest 1985; 45:471-6.\n3. Laurberg P et al. Scand J Clin Lab Invest 1987; 47:685-9.

Additional Info

Recognizes human Thyroglobulin. Has been shown to be useful in positive identification of thyroid carcinomas of the papillary and follicular types. Demonstration of thyroglobulin in a metastatic lesion establishes the thyroid origin of the tumor. Adenocarcinomas of non-thyroidal origin are not reactive.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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