Thyroid Hormone Receptor alpha (THRA, THRA1, THRA2, AR7, c-erbA-1, ERBA1, c-erbA-alpha, ERBA, EAR7, EAR-7, ERB-T-1, MGC000261, MGC43240, Nuclear Receptor Subfamily 1 Group A Member 1, NR1A1, V-erbA-related Protein 7)

Thyroid hormone receptor alpha (TR alpha), a NR1 Thyroid Hormone-Like Receptor, mediates the diverse actions of thyroid hormone in development, differentiation, and metabolism. Four protein isoforms are encoded by the same TR alpha genomic locus: TR alpha1 (410 aa) (Nakai et al. 1988), TR alpha2 (490 aa) (Nakai et al. 1988), TR deltaalpha1 (~154 aa), and TR deltaalpha2 (~237 aa); however, only TR alpha1 is able to bind the ligand (Lazar et al. 1988). TR alpha1 and TR alpha2 are products of alternative splicing of the primary TR alpha mRNA, whereas TR deltaalpha1 and TR deltaalpha2 arise from transcription of an internal promoter in intron 7 of the TR alpha gene. A truncated form of TR alpha, called p43 for 43kD, has been shown to regulate mitochondrial transcription Casas et al. 2003. Mice lacking all forms of TR alpha have been shown to be viable, but show reduced linear growth, delayed bone maturation, increased hypothermia, reduced thickness of the intestinal mucosa, lowered heart rates, and lowered serum T4. TR alpha has been linked to chronic fatigue (Englebienne et al. 2003).\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman surface epithelium\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5348-98P

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. J Endocrinol 2006 Oct. 191:221-8 Thyroid hormone-stimulated differentiation of primary rib chondrocytes in vitro requires thyroid hormone receptor {beta}. Rabier B, Williams AJ, Mallein-Gerin F, Williams GR, and Chassande O. 2. Biomarkers 2006 Nov. 11:524-37 Thyroid hormone-dependent gene expression as a biomarker of short-term 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) exposure in European common frog (Rana temporaria) tadpoles. Mortensen AS, Kortner TM, and Arukwe A. 3. J Mol Endocrinol 2006 Oct. 37:251-7 PGC-1{alpha} regulates the isoform mRNA ratio of the alternatively spliced thyroid hormone receptor {alpha} transcript. Thijssen-Timmer DC, Schiphorst MP, Kwakkel J, Emter R, Kralli A, Wiersinga WM, and Bakker O. 4. J Clin Endocrinol Metab 2006 Oct. Thyroid hormone signaling in human ovarian surface epithelial cells. Rae MT, Gubbay O, Kostigiannou A, Price D, Critchley HO, and Hillier SG. 5. Aquat Toxicol 2006 Sep. The bactericidal agent triclosan modulates thyroid hormone-associated gene expression and disrupts postembryonic anuran development. Veldhoen N, Skirrow RC, Osachoff H, Wigmore H, Clapson DJ, Gunderson MP, Van Aggelen G, and Helbing CC.

Additional Info

Recognizes human TR Alpha/NR1A1.