Thyroid Iodide Transporter (TIT)

Iodide (I-) is an essential constituent of thyroid hormones. Thyroid epithelial cells for the biosynthesis of thyroid hormones actively transport it. At the cellular level, Iodide transport is mediated by intrinsic membrane Na+/I+ symporter (NIS/TIT). Rat TIT/SLC5A (618aa; ~66kD; mouse 618aa; human 643aa) is predicted to contain 12 transmembrane domains. The N-terminus has a short extracellular domain, whereas C-terminus is predicted to be cytoplasmic. TIT is primarily expressed in thyroid but also detected in mammary gland and ovary. Its expression is reduced in tumors. Defects in TIT gene are associated with iodide transport defect (ITD), which is characterized by an inability of the thyroid to maintain concentration difference of readily exchangeable iodine between plasma and the thyroid gland. It is a cause of congenital hypothyroidism.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml\nWestern Blot: 1:1000-5000, 1-10ug/ml (ECL); A band of 95-110kD has been detected in brush border membrane (2).\nImmunohistochemistry (fixed): 2-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5610-01

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

References: 1. Dai, G., et al., Nature 379: 458-460 (1996). 2. Paire, A., et al., J. Biol. Chem. 272: 18,246-18,249 (1997). 3. Smanik, P.A., et al., BBRC 226: 339-345 (1996). 4. Saito, T., et al., J. Clin. Endocrinol. Metabol. 82: 3331-3336 (1997). 5. Fujiwara, H., et al., Nat. Genet. 16: 124-125 (1997). 6. Kosugi, S., et al., J. Clin. Endocrinol. Metabol. 84: 3248-3253 (1999). \nCitations: 1. Josefsson, M., et al., Acta Physiologica Scinavica 175(2): 129-137 (2002).

Additional Info

Recognizes rat Thyroid Iodide Transporter. No significant sequence homology is seen with glucose transporter or other Na+-dependent transporters.