Thyroid Peroxidase, Human, BioAssay(TM) ELISA Kit (TPO, TMA, Thyroid Microsomal Human Antigen)

The United States Biological Thyroid Peroxidase, Human, BioAssay(TM) ELISA Kit is intended for the detection and semi-quantitation of antibodies to microsomal in human serum. The assay is to be used to detect antibodies in a single serum specimen. \n\nAutoimmune thyroid disease is characterized by the presence of circulating autoantibodies directed to thyroid antigens. Hashimoto and Graves diseases are the best known of these diseases. The majority can be diagnosed by clinical presentation and their antibody profiles to thyroglobulin and thyroid microsomes.\n \nThyroglobulin is a water soluble glycoprotein that is involved in the storage and synthesis of thyroid hormones. The thyroid microsomal antigen has been shown to be the enzyme thyroid peroxidase (TPO). Antibodies to thyroglobulin and or microsomal antigen are present in most patients with goitrous thyroiditis (Hashimoto disease), atrophic thyroiditis (myxedema) and about 70-90% of Graves disease. Antibodies are also found in about half of the patients with primary hypothyroidism and thyrotoxicosis, and 10-20% of patients with simple goiters and thyroid tumors. There is also a relationship between thyroid antibodies and diabetes mellitus. Thyroid autoantibodies are present in about 6-7% of normals and their incidence increases with age.\n \nClassically, autoantibodies to thyroid antigens are detected by precipitation reactions, hemag- glutination and by immunoflourescence. However the tests are subjective and lack high sensitivity. ELISAs combine greater sensitivity, objective reading and ease of use. ELISAs have been developed and validated for detecting autoantibodies to thyroid antigens.\n\nKit Components:\nEach kit contains the following components in sufficient quantities to perform the number of tests indicated on the package label.\n1. Microtiter Plate: 1x96 wells\n2. Sample Diluent: 1x30ml\n3. Positive Control, High: 1x400ul\n4. Calibrator: 1x400ul\n5. Negative Control: 1x400ul\n6. Positive Control, Low: 1x400ul\n7. IgG, IgA, IgM (HRP) Goat x human: 1x15ml\n8. Wash Buffer, 20X: 1x50ml\n9. Tetramethylbenzidine (TMB): 1x15ml\n10. Stop Solution: 1x15ml\n\nStorage and Stability: \nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5399-02B

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Peter, J.B. 1981. Thyroid autoimmunity: in search of antibodies. Diagnostic Medicine. 4:19-25. 2. Davies, T.F., et al., Autoimmune Endocrine Disease, T.F. Davies, ed. Wiley, New York, NY. p. 127. 3. Wall, J.R. and T. Kuroki. 1985. Immunological factors in thyroid disease. Medical Clinics of North America. 69: 913. 4. Delespesse, G., et. al. 1972. Thyroid autoimmunity. In: Thyroiditis and Thyroid Function, P.A. Bastenie and A.M. Evans, eds. Pergamon Press, Oxford. p. 39. 5. Riley, W.J., et al., J. Pediati. 98:350-4. 6. Hawkins, B.R., et al., J. Clin Lab Immunol. 2:211-5. 7. Roman, S., et al., Clin. Chem. 30:246. 8. Goodburn, R., et al., J. Clin Path. 34: 1026-31. 9. Schardt, C.W., et al., J. Immunol Methods. 55: 155-168. 10. CDC-NIH Manual. 1993. In: Biosafety in Microbiological and Biomedical Laboratories, 3rd Edition. U. S. Dept. of Health and Human Services, Public Health Service. pp 9-12. 11. National Committee for Clinical Laboratory Standards. 1990. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture Approved Standard. NCCLS Publication H18- A. 12. Engvall, E., and P. Perlman. 1971. Enzyme-Linked Immunosorbent Assay, (ELISA) Quantitative Assay of Immunoglobulin G. Immunochemistry. 8: 871-874. 13. Engvall, E., and P. Perlman. 1971. Enzyme-Linked Immunosorbent Assay, ELISA. Peeters. H., ed. In: Protides of the Biological Fluids. Proceedings of the Nineteenth Colloquium, Brugge, Oxford. Pergamon Press. pp 553-556. 14. Engvall, E., et al., Biochem. Biophys. Acta. 251: 427-434. 15. Van Weeman, B. K. and A.H.W.M. Schuurs. 1971. Immunoassay Using Antigen-Enzyme Conjugates. FEBS Letter. 15: 232-235. 16. NCCLS. 1991. National Committee for Clinical LaboratoryStandard. Internal Quality Control Testing: Principles & Definition. NCCLS Publication C24- A. 17. http://www.cap.org/html/ftpdirectory/checklistftp. html. 1998. Laboratory General - CAP (College of American Pathology) Checklist (April 1998). pp 28-32. 18. NCCLS. 1997. National Committee for Clinical Laboratory Standard. Preparation and Testing of Reagent Water in the Clinical Laboratory. NCCLS Publication C3- A3.