Thyroid Peroxidase, Recombinant, Human (TPO, TMA, Thyroid Microsomal Antigen)

Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100kD molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.\nRecombinant Human TPO produced in SF9 is a glycosylated, polypeptide chain coding for the extracellular domain of native TPO, containing 834 amino acids and having a molecular mass of 92,872 dalton. Recombinant TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nImmunological Functions:\n1. Binds IgG-type human auto-antibodies.\n2. Auto-antibodies to TPO recognize conformation-dependent epitopes.\n3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).\n\nApplications: \nSuitable for use in ELISA and Western Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.15-0.375ug/ml (depending on the type of plate and coating buffer). Suitable for biotinylation and iodination. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5399-04R

Size

5ug

Form

Supplied as a liquid in 16mM HEPES, pH 7.6, 160mM sodium chloride, 0.08mM potassium iodide 20% glycerol.

Purity

(same/more than)95% (SDS-PAGE)

References

1. Exp. Oncol. 28(1): 70-74 (2006). 2. Prague Med. Rep. 106(4): 399-408 (2005). 3. Chem. Res. Toxicol. 19(3): 351-355 (2006). 4. Endocrinology 147(6): 3085-3092 (2006). 5. Prikl. Biokhim. Mikrobiol. 42(1): 98-105 (2006). 6. Autoimmunity 38(8): 605-611 (2005).