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Thyroid Peroxidase, Recombinant, Human (TPO, TMA, Thyroid Microsomal Antigen)

Cat no: T5399-10B

Thyroid Peroxidase, Recombinant, Human (TPO, TMA, Thyroid Microsomal Antigen)

Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of ~100kD molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.\n\nSource:\nRecombinant human TPO containing 834 amino acids from Sf-9 insect cells.\n\nMolecular Weight: \n~101kD\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5399-10B

Size

20ug

Applications

ELISA, WB

Form

Supplied as a liquid in 16mM HEPES, pH 7.6, 160mM NaCl, 0.08mM Kl, 20% glycerol.

Purity

~95% (SDS-PAGE). Chromatographically purified

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