Thyroid Stimulating Hormone (TSH, Thyrotropin Subunit beta, Thyrotropin beta Chain, Thyroid-stimulating hormone subunit beta, TSH-beta, TSH-B, TSHB)

Thyroid-stimulating hormone (TSH or thyrotropin), produced in human pituitary glands, has a molecular mass of 25kD. An Important indicator of thyroid function, TSH is used to monitor thyroid associated diseases. Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release.\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 1:50-1:500\nWestern Blot: 1:50-1:500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5400-43N

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from ammonium bicarbonate. No preservative added. Reconstitute with 50ul sterile ddH2O.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

References

1.Kasper, S. et al. Cell Tissue Res 325: 303-13

Additional Info

Recognizes beta subunit of human thyroid stimulating hormone (TSH) and the whole molecule. Negligible reactivity has been observed with human FSH, prolactin, human GH, alpha human CG, beta human CG and human ACTH. Species crossreactivity: fish.