Thyroid Transcription Factor 1 (TTF-1, Thyroid nuclear factor 1, Homeobox protein Nkx-2.1)

TTF-1 (38kD) is a member of the Nkx2 family of homeodomain-containing transcription factors. TTF-1 has been identified as a mediator of thyroid-specific gene transcription, and is involved in the activation of pulmonary-specific genes.2 TTF-1 is expressed in the epithelial cells of the lung and thyroid gland. TTF-1 is useful as part of a panel of antibodies used to differentiate lung and thyroid primary carcinomas from other carcinomas that metastasize to the lung such as breast and colon carcinomas3. It may also be useful as part of a panel of antibodies, including anti-calretinin, used to differentiate primary lung adenocarcinomas from mesotheliomas.4\n\nApplications:\nSuitable for use in Immunofluorescence, Immunocytochemistry and Immunohistochemistry (frozen and paraffin sections, after microwave pretreatment). Tumors Specifically Detected: Useful in the classification of thyroid and lung tumors.\n\nRecommended Dilution: \nImmunohistochemistry: 1:10-1:20\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nNormal thyroid or lung\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5405-02

Size

1ml

Applications

ICC, IF, IHC

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Holzinger A et al: Monoclonal antibody to thyroid transcription factor 1. Hybridoma 15, 49-53 (1996)

Additional Info

Reactive with human and rat. Recognizes a 40kD protein, identified as thyroid transcription factor 1 (TTF-1), selectively expressed in the lung, thyroid and diencephalon. The antigen has been localized in the nucleus.