Thyroid Transcription Factor 1 (TTF1, NKX2-1, NKX Homeobox Protein)

Thyroid Transcription Factor 1 (TTF1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF1 plays an important role in differentiation and morphogensis of the developing thyroid, lung, and ventral forebrain (2). TTF1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence(Immunocytochemistry): 1:50\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137503

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Civitareale, D. et al. (1989) EMBO J 8, 2537-42. 2. Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35. 3. Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61. 4. Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62. 5. Yoshida, A. et al. (2011) Lung Cancer 72, 309-15. 6. Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.

Additional Info

Recognizes endogenous levels of total human Thyroid Transcription Factor 1. Species Crossreactivity: mouse, rat.