Thyrotropin (TSH) Receptor (hTSHR I, hTSHR-I, LGR3, MGC75129, Thyroid Adenoma Hyperfunctioning, Thyroid Carcinoma with Thyrotoxicosis, Thyroid Stimulating Hormone Receptor, TSH R, TSHR, TSH-R, Thyrotropin Receptor I)

The thyrotropin receptor (or TSH receptor) is a gene (and associated protein) which responds to thyroid-stimulating hormone (also known as "thyrotropin", and stimulates the production of thyroxine (T4) and triiodothyronine (T3). The TSH receptor is a member of the G-protein coupled receptor superfamily of integral membrane protein. It is primarily found on the surface of the thyroid epithelial cell. It should not be confused with "thyrotropin-releasing hormone" (TRH) or "thyrotropin-releasing hormone receptor" (TRHR). The TSH receptor is found mainly on thyroid follicular cells. Stimulation of the receptor increases T3 and T4 production and secretion.\nStimulating antibodies to this receptor mimic TSH and cause Graves' disease. The thyrotropin receptor has been reported in adipose, adrenal, brain, eye, heart, kidney, skin, thymus, and thyroid. ESTs have been isolated from brain, placenta, and thyroid libraries.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500. Detects a 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSRH respectively, in transfected Sf9 cell extract.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5430-05A

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Prc

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

This product has been used in:Seetharamaiah GS et al. Generation and characterization of monoclonal antibodies to the human thyrotropin (TSH) receptor: antibodies can bind to discrete conformational or linear epitopes and block TSH binding. Endocrinology 136:2817-24 (1995). <PUBMED:7540542> Patibandla SA et al. Differential reactivities of recombinant glycosylated ectodomains of mouse and human thyrotropin receptors with patient autoantibodies. Endocrinology 138:1559-66 (1997). <PUBMED:9075716>Wagle NM et al. Thyrotropin receptor-specific antibodies in BALB/cJ mice with experimental hyperthyroxinemia show a restricted binding specificity and belong to the immunoglobulin G1 subclass. Endocrinology 136:3461-9 (1995). <PUBMED:7628382>Akamizu T et al. Molecular studies on thyrotropin (TSH) receptor and anti-TSH receptor antibodies. Endocr J 42:617-27 (1995). <PUBMED:8574284>

Additional Info

Species Crossreactivity: Crossreacts with Human and porcine.