TIA1 (TIA 1, TIA-1, TIA1 Protein, Cytotoxic Granule-associated RNA Binding Protein, Nucleolysin TIA1 isoform p40, Nucleolysin TIA-1 Isoform p40, p40 TIA-1, RNA Binding Protein TIA1, RNA Binding Protein TIA-1, TIA-1 Cytotoxic Granule-associated RNA Binding

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15kD protein that is thought to be derived from the carboxyl terminus of the 40kD product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.\n\nApplications: \nSuitable for use in Peptide ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nPeptide ELISA: 1:128,000\nWestern Blot: 0.1-0.3ug/ml. Band of ~40kD was observed in lysates of Jurkat cell line. An additional band at 80kD and a very strong 26kD band was also observed. These bands were successfully blocked by incubation with the immunising peptide.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5461-95

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in Tris-saline, pH7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Yang F, et al. Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1. Mol Cell Biol. 2006 Dec;26(23):8803-13.

Additional Info

Recognizes human TIA1. Species Crossreactivity: mouse, rat and canine