TID 1 (Tumerous Imaginal Discs 1)

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumerous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as co-chaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents such as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro-apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals. \n\nApplications: \nSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nImmunoprecipitation: 2ug/mg protein lysate (Use Protein G). Co-precipitates mitochondrial Hsp70 \nWestern Blot: 1-2ug/ml for 2 hrs at RT \nOptimal dilutions to be determined by the iresearcher.\n\nPositive Control: \nHeLa or Jurkat cells \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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