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Tie2, phosphorylated (Tyr1102) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Cat no: T5498-70D

Tie2, phosphorylated (Tyr1102) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Tie2 (also known as TEK) is an angiogenic receptor tyrosine kinase required for the later stage of blood vessel maturation. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation of Tie2 at Y1102 results in the activation of PI 3-kinase. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested\n\nRecommended Dilutions:\nWestern Blot: 1ug/ml\nImmunohistochemistry: 5-15ug/ml detected Tie2 in immersion fixed paraffin embedded sections of mouse embryonic brain and human placenta.\nOptimal dilutions should be determined by each laboratory for each application. \n \nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5498-70D

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Tie-2 when phosphorylated at Y1102. \nSpecies Crossreactivity: mouse when phosphorylated at Y1100.

Read more on Supplier website

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info
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