Tie2, phosphorylated (Tyr992) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

TIE2, phosphorylated (pTyr992) (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 TEK, Tyrosine Protein Kinase Receptor) is a receptor tyrosine kinase (RTK). It is expressed almost exclusively on endothelial cells. It is critical for vasculogenesis during development and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis (1-3). The ligand for Tie has yet to be identified. A family of ligands known as the angiopoietins have recently been shown to bind Tie2. These ligands appear to have opposing actions as Angiopoietin-1 Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2. Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation (4, 5). The phosphorylated Tie2 associates with different downstream signaling components. Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase and p56Dok-2 can interact with TIE2 in a phosphotyrosine- dependent manner through their SH2 or PTB domains (6, 7). Tyrosine 992 is located on the putative activation loop of Tie2 and is a major autophosphorylation site (8).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5498-70A\n

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Ward, N.L., Dumont, D.J., Semin. Cell Dev. Biol. 13: 19-27 (2002). 2. Jones, N., Dumont, D.J., Cancer Metastasis Rev. 19: 13-17 (2000). 3. Partanen, J., Dumont, D.J., Curr. Top. Microbiol. Immunol. 237: 159-172 (1999). 4. Ellis, L.M., et al., Oncology 16: 31-35 (2002). 5. Koh, G.Y., et al., Exp. Mol. Med. 34: 1-11 (2002). 6. Jones, N., et al., J. Biol. Chem. 274: 30,896-30,905 (1999). 7. Jones, N., et al., Mol. Cell. Biol. 23: 2658-2668 (2003). 8. Murray, B.W., et al., Biochem. 40: 10,243-10,253 (2001).

Additional Info

Detects overexpressed levels of Tie2 only when phosphorylated at tyrosine 992 (Mr 160kD). Does not crossreact with other major activated RTKs. Species sequence homology: mouse 100%.