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Tie2 Receptor (Tunica Internal Endothelial Cell Kinase Receptor, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Cat no: T5498-80

Tie2 Receptor (Tunica Internal Endothelial Cell Kinase Receptor, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Tie2/Tek is a receptor tyrosine kinase (RTK) expressed almost exclusively on endothelial cells and critical for vasculogenesis during development and could be important for maintaining endothelial cell survival and integrity in adult blood vessels as well as tumor angiogenesis. The ligand for Tie has yet to be identified. A family of ligands known as the angiopoietins have recently been shown to bind Tie2. Interestingly, these ligands appear to have opposing actions as Angiopoietin-1 (Ang1) and Angiopoietin-4 (Ang4) stimulate tyrosine phosphorylation of Tie2, whereas Angiopoietin-2 (Ang2) and Angiopoietin-3 (Ang3) can inhibit this phosphorylation. The phosphorylated Tie2 associates with different downstream signaling components. Grb2, Grb7, Grb14, Shp2, the p85 subunit of phosphatidylinositol 3-kinase and p56Dok-2 can interact with Tie2 in a phosphotyrosine-dependent manner through their SH2 or PTB domains.\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5ug/ml, detects 20ng recombinant Tie-2. Recognizes a band at ~140kD\nFlow Cytometry: 10ug/ml \nImmunoprecipitation: 10ug/ml of lysate \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nPlacenta and lung tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5498-80

Size

100ug

Applications

ELISA, FC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% Bronidox as preservative, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Runting, A.S., et al., Growth Factors 9: 99

Additional Info

Recognizes mouse Tie2 Receptor.

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